In a recent paper in the journal PLOS ONE the authors describe an approach based on bioluminescence resonance energy transfer or BRET to monitor dopamine receptor activation. As with other BRET approaches this assay monitors a protein-protein interaction which in this case is indicative of dopamine receptor activation. A binding peptide was linked to the recently engineered NanoLuc luciferase and a protein that binds to this peptide was linked to the Venus fluorophore. Treatment with agonist leads to association between the peptide and protein which brings NanoLuc into proximity of the Venus leading to a BRET signal.
|"Dopamine Electron Map" by Jaelkoury - Rendered on Spartan. |
Licensed under Creative Commons Attribution-Share Alike 3.0
via Wikimedia Commons http://commons.wikimedia.org
For more information on the POLARstar Omega and other microplate readers from BMG LABTECH please visit: http://www.bmglabtech.com/
Article citation: J.C. Octeau, et al. "G Protein Beta 5 Is Targeted to D2-Dopamine Receptor-Containing Biochemical Compartments and Blocks Dopamine-Dependent Receptor Internalization" PLoS One. 2014; 9(8)