This work appears in the article entitled: 'Targeted p16Ink4a epimutation causes tumorigenesis and reduces survival in mice'. The article describes the modification of the promoter for p16, an important tumor suppressor gene, such that DNA methyltransferases were attracted to this site and hypermethylation occured, with the anticipated result of p16 gene silencing. The authors provide a variety of controls to indicate that the loss of p16 is due to hypermethylation of its promoter and show that the result of this loss was a higher incidence of cancer and reduced rate of survival.
|This image shows a DNA molecule that is methylated|
on both strands on the center cytosine.
DNA methylation plays an important role for epigenetic
gene regulation in development and cancer.
by Christopher Bock
For more information on how microplate readers from BMG LABTECH can help with epigenetic or other screening assays please visit our website: http://www.bmglabtech.com/
Some information for this post was obtained from the Science Daily article: 'Epigenetic changes can drive cancer, study shows'
Original article citation: Da-Hai Yu, Robert A. Waterland, Pumin Zhang, Deborah Schady, Miao-Hsueh Chen, Yongtao Guan, Manasi Gadkari, Lanlan Shen. Targeted p16Ink4a epimutation causes tumorigenesis and reduces survival in mice. Journal of Clinical Investigation
BMG LABTECH application notes:
247 Screening for Histone Deacetylase (HDAC) Active Compounds