The BMG LABTECH application note entitled: 'Lonza’s Kinetic Kit for Endotoxin Detection Using the FLUOstar OPTIMA and MARS Data Analysis' describes an approach that allows users to confidently detect LPS contamination. Lonza's kinetic kit contains a proenzyme that is activated in the presence of endotoxin which cleaves a colorless peptide to release p-nitroaniline which is detectable at 405 nm.
|Signal curves for several endotoxin samples taken directly|
from MARS data analysis software.
Kinetic signal curves for standards and unknown could then be produced and analyzed in the MARS data analysis software that is provided with all BMG LABTECH microplate readers. The result of the analysis is a standard curve that can be used to reliably determine the concentration of LPS in the unknown samples.
For the details of this and other applications suitable for use on BMG LABTECH microplate readers please visit the Applications Center at our website: www.bmglabtech.com