Thursday, June 20, 2013

Dual Luciferase Reporter (DLR) Assay Certification on the CLARIOstar® using LVF monochromators™

The Dual Luciferase Reporter Assay is an essential tool used in the studies of transcriptional regulation. As such, it important that the capability of existing microplate readers is confirmed for the performance of this assay. This is the basis for the DLReady certification program. BMG LABTECH is proud to say that the new CLARIOstar has passed this certification process and the results that led to this certification are presented in BMG application note #239.

Dual Luciferase Reaction: Luciferase Assay Reagent II
(LAR II) is injected in step 1 to initiate the Firefly
luciferase reaction. In the second step the injection of Stop
and Glo® step quenches the Firefly reaction while starting
the reaction for Renilla Luciferase.
In order to attain DLReady certification 3 criteria must be attained based on three tests. 1) A quenching experiment which indicates that the Stop and Glo injection sufficiently suppresses the reaction produced by Luciferase Reagent II. 2) A consistency experiment that shows that replicates at different concentrations of Firefly and Renilla luciferase deviate by less than 5% CV. 3) A tubing adsorption experiment that shows that reproducible results are obtained after waiting for 10 minutes. The CLARIOstar exceeded all criteria and has attained official DLReady certification!

For more information on the CLARIOstar and other DLReady microplate readers from BMG LABTECH please visit our website: www.bmglabtech.com

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