Thursday, April 11, 2013

Did you know: you can monitor the activity of three enzymes in one microplate well

Application note 235, now available on the BMG LABTECH website, describes the use of the multifunctional microplate reader the FLUOstar Omega to perform a screen for inhibitors of metalloaminopeptidases. Screening for these inhibitors will hopefully reveal new antimalarial agents.

Malaria is a mosquito-borne disease which results when a host organism is infected with the protozoan parasite Plasmodium  vivax or other members of the genus Plasmodium. While the disease is restricted to the tropics it still accounts for nearly one million deaths per year. Therefore the search for new treatments is an area of intense research.

Malarial sporozites develop inside oocytes and are
released in large numbers into the hemocoel of
Anopheles stephensi mosquitoes. This false-colored
electron micrograph shows a sporozite migrating through
the cytoplasm of midgut epithelia.
Image by Ute Frever, false color by Margaret Shear
During an infection metalloaminopeptidases digest host hemoglobin and are essential for parasite survival. So, finding inhibitors of these enzymes could result in novel therapeutic options.

As with all screening strategies it is important to decrease the amount of compound used. To this end the current application note details a technique that can monitor activity of three metalloaminopeptidases at the same time.

Using the multifunctional capabilities of the FLUOstar Omega from BMG LABTECH the breakdown of substrates was monitored using fluorescence and absorbance modes. Thus kinetic assessment of enzyme activity could be monitored and inhibitors could be screened.

To find out more about the multifunctional capacity of the FLUOstar Omega and the other microplate readers from BMG LABTECH please visit our website:  http://www.bmglabtech.com/

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