Thursday, January 10, 2013

Did you know that non-binding plates should not be used in the NEPHELOstar Plus because it increases light-scattering and thus the blank?

The NEPHELOstar Plus uses a high-intensity light source which is directed through a sample. Particles in the sample are detected based on the amount of light scatter that occurs. This detection is approximately 30 times more sensitive than readers that detect a reduction in direct light. The increased sensitivity makes the NEPHELOstar Plus useful for a number of applications including: drug solubility screening and growth curve monitoring for microbes.

 However, the high sensitivity can make this instrument susceptible to detecting high background when anything, besides the sample, that can scatter light is also present. This includes the substances and other coatings that are used to make non-binding microplates effective. In our tests, non-binding plates increased the blank 90 times that seen with non-coated plates. To obtain great results using the NEPHELOstar Plus stick with untreated plates of up to 384 wells for your assays.

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