Tuesday, January 15, 2013

Did you know cell based screens can be performed on as few as 250 cells/well?

It is now feasible to perform cell-based assays in sub micoliter volumes using as few as 250 cells due to improvements in assay sensitivity, liquid handling and microplate reader technology. The PHERAstar FS from BMG LABTECH, The Microplate Reader Company, was used in conjunction with technologies from DiscoveRx, and Labcyte to show that analysis of G-protein coupled receptor function could be performed in 3456 well plates. Specifically, suspension CHO cells at different densities, which express the dopamine receptor, were tested for their ability to respond to agonist. Results indicate that 250 cells in less than 1 microliter exhibit a clear response.

3456-well Protocol Optimization. PathHunter® DRD2L cells 100 or 250 cells/well (in suspension) were added to the AURORA 3456 well plates in 0.5 or 1 μL volumes via the Echo® instrument. Compound (5nl of 100X in DMSO) was added to the cells plated in 0.5 µl (10 nl for the cells plated in 1µl) via the Echo® instrument.

With high through put assays of this type it will be possible to perform more screens with lower reagent costs.

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