Apoptosis is a multistage process during which activity of caspase enzymes fluctuates, DNA becomes fragmented and phosphatidyl serine is transferred to the outside of the cell membrane. Apoptosis is a common pathway that can go wrong in many cancers and it is a highly researched area for different cell types. Common methods for apoptosis analysis include:
- Caspase activity assay, either colorimetric, fluorescent, luminescent or antibody based
- TUNEL assay, based on DNA fragmentation.
- Annexin-V assay, based on the binding of dye or fluorescently-conjugated Annexin-V to phosphatidyl serine which has translocated to the cell membrane exterior during apoptosis.
To learn more about this assay on the SPECTROstar Nano, please see Application Note 228: Biocolor's APOPercentage Apoptosis Assay™ on BMG LABTECH's SPECTROstar Nano plate reader. Also, learn more about Bicolor here.
|Colorimetric Quantification. Graph Showing Effect of Hydrogen Peroxide (0 – 10mM) on CHO Cells. Results expressed as mean absorbance for triplicate wells ± S.E.M. (n = 3). Exposure time to H2O2 was 4 hours.|