Tuesday, November 20, 2012

Did you know that a BMG LABTECH microplate reader can take some of the workload off of your High Content Screening (HCS) system?

High content screening allows researchers to see the phenotype of a cell and how it is affected by various treatments. Information about cell shape or where two fluorescent proteins co-localize isn’t information that can be obtained many other ways. However, some changes—cell motility, cell death, fluorescent protein expression levels—might require more information than a standard plate reader can provide but don’t require the full imaging capability of an HCS system.

Readers like BMG LABTECH’s PHERAstar FS can focus precisely from the bottom of the well and measure cell-based assays in scanning mode, taking hundreds of data points per well to create a fluorescence intensity map. It isn’t possible to see what’s happening in individual cells, but it’s easy to see cell coverage and uniformity, or where in the well expression is happening. An assay that can take two hours on an HCS system would take only eight minutes on the PHERAstar FS, meaning that researchers can collect more data per hour and that expensive HCS equipment is free to be used for methods that truly require its capabilities.
This figure shows the well scanning capabilities of the PHERAstar FS
using a 384-well plate seeded with cells expressing different levels of GFP.
 Here is an application note that describes in detail how the PHERAstar FS outperforms a leading CCD device in HTRF® mode:
HTS Instrument Discovers Low Affinity Inhibitors of the Inositol Phosphate (IP) Signaling Pathway.

For more information on Developing and Optimizing Cell-based Microplate Assays, join us for our webinar on Wednesday, November 28. More information can be found here:
http://www.bmglabtech.com/webinar/cell-based-assays.cfm

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