One main difference between these new dual glow reporter assays and earlier dual glow assays like DLR™, is that the two different luminescent signals can be differentiated at different wavelengths, rather than at different time points. This enables higher throughput because there is no need for injection and no need for a minimal measurement time, the only limitation is in the instrumentation.
Larger bandwidth filters can capture more signal.
In addition, since these luminescent signals are not as bright as fluorescent signals, it benefits from collecting a larger area of the emission signal at each wavelength. Thus microplate readers with the ability to measure broad bandwidths (50-100 nm), like the POLARstar Omega and PHERAstar FS because they use longer bandwidth filters, will be more sensitive in these assays.
Learn more about it here, Wavelength Based Dual Glow Reporter Genes.