Friday, October 19, 2012

Focus On Mitochondrial Toxicity – Simple Intracellular assay to test oxygen consumption

Mitochondria are one of the main places in cells were energy is produced via aerobic respiration. Mitochondrial toxicity can occur when drugs or diseases affect the mitochondria and prevent the proper production of energy via oxygen consumption. Therefore a method is needed to test mitochondrial function and toxicity, especially when trying to design potential new drugs.
One way to measure mitochondrial function is to measure oxygen consumption, but traditional methods are limited by low throughput, by using specialized equipment, and by measuring only extracellular oxygen.

The MitoXpress®-Xtra assay from Luxcel Biosciences overcomes these limitations by providing a phosphorescent based method that can measure intracellular oxygen consumption using the time-resolved detection found in many multimode microplate readers. When used with the Atmospheric Control Unit (ACU)  on the FLUOstar Omega, a real-time monitoring system can be created that measures the change in intracellular oxygen in a cell monolayer (see Application Note 223).

Find out more about the MitoXpress®-Xtra assay on our website: http://www.bmglabtech.com/technology/mitoxpress.cfm.

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