Dr. Lefkowitz’s lab has used a BMG LABTECH microplate reader to measure amongst other things, intracellular calcium flux, cAMP accumulation, and b-arrestin recruitment, which are all downstream effects of GPCRs.
You can find out more about some of these publications here:
- Arresting a Transient Receptor Potential (TRP) Channel (http://www.jbc.org/content/285/39/30115.full.pdf)
- Quantifying Ligand Bias at Seven-Transmembrane Receptors (http://molpharm.aspetjournals.org/content/80/3/367.full)
- β-Arrestin1 mediates nicotinic acid–induced flushing, but not its antilipolytic effect, in mice (http://www.jci.org/articles/view/36806)
- A unique mechanism of β-blocker action: Carvedilol stimulates β-arrestin signaling (http://www.pnas.org/content/104/42/16657.full)