Thursday, September 6, 2012
Thursday Application – A Calcium Bioluminescence Assay: A New Research Based Application Video
A research based application video entitled, A Calcium Bioluminescence Assay for Functional Analysis of Mosquito (Aedes aegypti) and Tick (Rhipicephalus microplus) G Protein-coupled Receptors, has been published on JoVE, the Journal of Visualized Experiments, and it uses the NOVOstar microplate reader. This video publication shows in detail how researchers at Texas A&M produced a stable cell line in mammalian CHO cells to investigate GPCRs from two different anthropods, the southern cattle tick Boophilus microplus (Canestrini), and the mosquito Aedes aegypti (Linnaeus). The NOVOstar microplate reader was used to study the GPCR activated calcium response of these stably transfected cell lines using a bioluminescent signal. As the authors note:
“This method has three primary applications. First, the technique can be applied for receptor deorphanization through ligand activity measurements. Second, the assay can resolve ligand-receptor structure-activity relationships (SAR). Third, the methods can be used in drug discovery. Furthermore, this protocol can be used to study the activity of agonists or antagonists on almost any GPCR.”
View all BMG LABTECH videos here and view the research based application videos here.