Thursday, September 13, 2012

Inside Applications: HTS Instrument Discovers Low Affinity Inhibitors of the Inositol Phosphate (IP) Signaling Pathway

IP One Assay Pronciple Point Several low affinity drug 'hits' were only found by the PMT-based reader and not the CCD-based reader
Point PHERAstar FS performs more than twice as fast as the CCD-based reader
Point PHERAstar FS shows superior assay quality parameters such as Z', DeltaF% and assay window
The IP-One HTRF® assay from Cisbio measures the lower response second messenger inositol 1,4,5-triphosphate (IP3). This assay was performed on two different HTS microplate readers with different detection technologies. The data shows that low affinity compounds which were not discovered with a leading HTS CCD camera based imaging microplate reader were readily resolved with the PMT based PHERAstar FS microplate reader from BMG LABTECH.

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