FAQ: Why do you need different microplates when measuring DNA and protein using 260 and 280 nm absorbance measurements, respectively?
When performing absorbance measurements in the UV range (<400 nm), UV clear microplates are needed for accurate measurements. The most common microplates are made of polypropylene or polystyrene and these materials absorb light in the UV range. Therefore, DNA/protein quantitation that require measurements at 260/280 nm need a material that will not absorb UV light.
There are several types of UV-transparent microplates, including: