Monday, August 13, 2012

FAQ: When using the Atmospheric Control Unit (ACU)for cell-based assays, what are the benefits of having independent control of both O2 and CO2 in your microplate reader, i.e. two different gas ports with separate sensors?

Control of O2 and CO2 in a microplate reader
Fig. Kinetic oxygen probe showing the response of
the cell monolayer to decreasing ambient oxygen.
This is possible with independent control of CO2 and O2. 
Cell-based assays require a mixture of CO2 and O2, however the amount of each gas that is needed is different for different types of cells, i.e. bacterial cells require different concentrations than mammalian or fungal cells. Using independently controlled gas supplies means that users have true flexibility as they can work with many different cellular organisms that require different conditions using the same gas supplies. In addition, manual ramping can be achieved to simulate disease conditions such as hypoxia, ischemia, and reperfusion without the need to change the gas supply (see Figure). Finally, using individual gas supplies is way more cost effective than using pre-mixed gases, on average costing 5-6 times less.

Learn more about BMG LABTECH’s Atmospheric Control Unit (ACU) and independent gas control here: The ACU is available on BMG LABTECH Omega Series of microplate readers.

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