Tuesday, August 21, 2012

Did you know that the Omega Microplate Readers with Advanced Assay Technology are also HTRF® Certified?

HTRF® is a TR-FRET based technology from Cisbio Bioassays that uses the principles of both TRF and FRET. The HTRF® donor fluorophore is either Europium cryptate (Eu3+ cryptate) or Lumi4™-Tb (Tb2+ cryptate), fruit of a recent collaboration with Lumiphore Inc..

HTRF® is a highly flexible chemistry and has been successfully used to measure molecular complexes of many different sizes. This includes assessment of small phosphorylated peptides, immunoassays for quantifying large glycoproteins such as thyroglobulin, receptor tyrosine kinase activity using membrane preparations and indirect detection (via secondary antibodies) of tagged complexes such as CD28/CD86 binding.

BMG LABTECH's PHERAstar Series and Omega Series are both HTRF® compatible certified. BMG LABTECH has also published numerous application notes that demonstrate the use of HTRF® technology with microplate readers. For more information visit: http://www.bmglabtech.com/technology/htrf.cfm

The fluorescence ratio associated with HTRF® readout is a correction method developed by CIS bio international and covered by the US patent 5,527,684 and its foreign equivalents. The dual simultaneous fluorescence time-resolved measurement is covered by CIS bio international’s US patent 6,352,672 and its foreign equivalents. CIS bio international has granted BMG LABTECH a license for the promotion of these methods with its microplate readers. The application of said methods is strictly limited to the use of HTRF® reagents and technology, excluding any and all other TR-FRET technologies.

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