Friday, June 29, 2012

Focus on Reagent Injectors – Injection at the Point of Measurement

For advanced assays on microplate readers, such as calcium or ion flux or gene transcription experiments, it is necessary to initiate the reaction with reagent injectors. Since these assays have quickly fluctuating or short lived signals, the microplate reader must be able to inject into a well of a microplate and then be able to quickly or instantly measure that well after injection.

A nice feature on BMG LABTECH microplate readers is Injection at the Point of Measurement. That is, as the reagent is being injected into the microplate well from the top, the specialized optics are measuring the signal from the bottom of plate at the same time. Therefore, none of the signal is lost on BMG LABTECH microplate readers. The signal can be captured as quickly as 50 measurement points a second, or once every 20 microseconds. If a microplate reader does not have Injection at the Point of Measurement, it can take several seconds after injection before the first measurement is read. If your assay is finished in seconds, these beginning measurement points will be lost. With BMG LABTECH microplate readers, this is not a concern.

Some common assays that require injection include:
  • Calcium or sodium flux
  • Ion voltage channels
  • Dual Luciferase Assay or DLR®
  • ORAC antioxidant assay
  • Enzyme kinetics
  • Flash luminescence or luciferase assay 
Learn more about reagent injection on BMG LABTECH microplate readers here:

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