|Fig. 3: Fluorescent ligand binding at |
human adenosine A1 receptors,
and displacement with the unlabelled
A1-selective competitor DPCPX.
In a new application note, we show how the adjustable focal height feature and the advanced Direct Optic Bottom Reading of the PHERAstar FS plate reader from BMG LABTECH allows for the quantification of fluorescent ligand binding to live, adherent cells in an HTS format. Using CellAurora’s GPCR fluorescent ligands for different GPCRs, IC50 pKi plots for a 96-well microplate were obtained in read times as fast as 2.5 minutes, producing a Z’ factor greater than 0.60. This is in stark contrast to HCA assays, which can take around 60 minutes to achieve the same endpoint.
Click here for the application note: http://www.bmglabtech.com/application-notes/fluorescence-intensity/gpcr-cellaura-pherastar-fs-227.cfm.