Thursday, June 21, 2012

Applications Thursday: Measuring Epigenetic Markers with BellBrook Labs’ Transcreener® Competitive Immunoassays

Most enzymatic reactions produce a byproduct that accumulates overtime as the reaction proceeds. Taking advantage of this byproduct accumulation, Bellbrook Labs has created a series of generic competitive immunoassays that can measure a plethora of biological processes. Their first such assay measures the accumulation of ADP, which can be used to monitor the activity of kinases, ATPases, or helicases. More recently, their newer AMP/GMP Transcreener® assay is able to measure the activity of epigenetic enzymes such as acetyltransferase and methyltransferase.


Using the PHERAstars series of HTS microplate readers, all of the Transcreener® assays were shown to produce large assay windows and high Z’ values at various times during the enzymatic reactions (i.e. 2, 5, 10 and 20% conversion). Please consult BMG LABTECH’s application note which details how to perform the Transcreener® assay on a BMG LABTECH microplate reader, such as the PHERAstar: Methyltransferase, Acetyltransferase, Kinases, and GTPases Can All Be Measured with Transcreener® Assays

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