Since the 1970, fungal infections have been on the rise and the best way to treat fungaemia is to detect infection as early as possible. In this proposal, a new method is highlighted that might be able to determine fungal infection earlier than the culture-based detection methods. Using the SPECTROstar Nano, microbial growth of fungi will be monitored from patient samples in 96-well microplates. Furthermore, microbial mass will be determined with SPECTROstar Nano in order to standardize DNA extraction. Lastly, PCR-ELISAs will be performed with the SPECTROstar Nano to determine which fungal species (dermatophyte or candida) is present in each patient sample.
|Photo by: David O Morgan|
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