Thursday, June 7, 2012

Applications Thursday: - High-throughput determination of yeast and dermatophyte species using the SPECTROstar Nano

Runner Up in BMG LABTECH's SPECTROstar Nano competition: Dr. Cornelia Wiegand, Universitätsklinikum Jena, Germany - High-throughput determination of yeast and dermatophyte species using the SPECTROstar Nano

Since the 1970, fungal infections have been on the rise and the best way to treat fungaemia is to detect infection as early as possible. In this proposal, a new method is highlighted that might be able to determine fungal infection earlier than the culture-based detection methods. Using the SPECTROstar Nano, microbial growth of fungi will be monitored from patient samples in 96-well microplates. Furthermore, microbial mass will be determined with SPECTROstar Nano in order to standardize DNA extraction. Lastly, PCR-ELISAs will be performed with the SPECTROstar Nano to determine which fungal species (dermatophyte or candida) is present in each patient sample.

Photo by: David O Morgan
“It can be expected that the proposed method for high-throughput determination of dermatophyte and yeast species using the SPECTROstar Nano will considerably simplify sample handling in daily laboratory practice by using one instrument for measurement of microbial growth, DNA quantification and PCR-ELISA read out. Hence, compared to traditional culture-based fungal detection methods, a reduction in time and handling steps can be assumed.”

Visit our website to read the entire paper: http://www.bmglabtech.com/promotion/dna-extraction-quantification.cfm

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