Monday, May 28, 2012

FAQ: What are the most common ELISA conjugate/substrate combinations and what are their appropriate absorbance wavelengths?

ELISA Enzyme and Substrate Systems 

Reading wavelength (nm)
Enzyme Conjugate label
Substrate
Kinetic or Nonstopped
End Point or Stopped
Horseradish peroxidase (HRP)
OPD (o-Phenylenediamine)
450
492
TMB (3,3′,5,5′-Tetramethylbenzidine)
630
450
 
ABTS
((2,2'-Azinobis [3-ethylbenzothiazoline-6-sulfonic acid])
414
414
Alkaline Phosphatase (Alk-phos)
pNPP
(p-Nitrophenyl Phosphate)
405
405
β-Galactosidase (β-gal)
ONPG
(o-Nitrophenyl-ß-D-galactopyranosidase)
420
420
Urease
Urea bromocresol
590
590

Here you can find a list of BMG LABTECH instruments that can perform absorbance measurements including ELISA: http://www.bmglabtech.com/products/microplate-reader/absorbance.cfm

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