Friday, April 20, 2012

Focus on HTS Library Compound Management

When managing an HTS compound library two experiments are performed routinely, testing for compound purity and testing for compound solubility. BMG LABTECH has two microplate readers that make these two experiments simple.

I)    Compound Purity is Determined Using Ultra-fast, Full Spectrum Analysis on the SPECTROstar Nano
To avoid contamination when using an HTS compound library, a working library is often created in a 384-well plate. Before (and during) use of this working library, the purity and concentration of the compounds are confirmed with a UV-Vis absorbance spectrum.
With the SPECTROstar Nano, a full absorbance spectrum for each well is captured (220–1000 nm at a 1 nm resolution) in less than 1 second/well, which amounts to less than 6 minutes for a 384-well microplate. Using a monochromator with 10 nm increments, this could take more than six hours per 384-well plate. In addition to being more than 60 times faster the resolution is 10 times better.

II)    Compound Solubility is Determined By Measuring Light-Scattering on the NEPHELOstar Plus Nephelometer

When determining the best concentration of compounds to use in an experiment, as well as determining maximum bioavailability upon dosing, the solubility points of the different compounds in an HTS library need to be determined.

With the NEPHELOstar Plus laser-based nephelometer, light-scattering is used to determine compound solubility in a microplate. Having a larger dynamic range than O.D. turbidity measurements, nephelometery allows for easy and accurate calculation of compound solubility points.

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