|Histone modifications caused by HDAC are strongly related to epigenetic regulation of transcription|
|HDAC inhibitors (HDIs) are a possible strategy in cancer therapy|
|Fluorescent HDAC activity and inhibitor assay was performed on a POLARstar OPTIMA microplate reader from BMG LABTECH|
Post-translational histone modifications like acetylation play an important role in the epigenetic regulation of transcription. Determining the activity of recombinant HDAC1 using the POLARstar OPTIMA microplate reader from BMG LABTECH offers high precision and performance. Using Boc-Lys(Ac)-AMC as fluorescent substrate HDAC activity and inhibitor assays can be performed over a wide concentration range.