FAQ: When should orbital averaging be used as opposed to well scanning?
Orbital average and well scan are mainly used for cell based assays or assays that are not homogenously distributed in the well. A center endpoint reading excites and measures only in the center of the well, giving one number. An orbital average reading excites and measures in a definable orbit around the well, giving one averaged number. A well scan reading excites and measures in a definable grid pattern in each well, giving multiple measurements per well. Many things can be done with well scan data such as setting thresholds and creating three dimensional graphs of each well.
For more information on well scanning and obital averaging, visit: http://www.bmglabtech.com/technology/wellscanning.cfm
Quantify Uneven Well Distribution in Cell-based Assays