Tuesday, February 14, 2012

Did you know that a vitamin E analog is often used as standard substance in the ORAC assay?

Signal curves for different Trolox® concentrations (red graphs)
and a blank without Trolox (blue graph) recorded on the
PHERAstar FS in 384-well format.
The curves were normalized to 100 %.
The 100 % value is the maximum value that is obtained
directly after injection of AAPH.
The water-soluble vitamin E analog 6-Hydroxy-2,5,7,8-tetra-methylchroman-2-carboxylic acid has thankfully a short name: Trolox®. This substance is often used as a standard and all sample results are expressed as Trolox® equivalents (TE).

In our application note 197, we describe the application of the ORAC assay on a POLARstar Omega and PHERAstar FS using Trolox® (a water-soluble analogue of vitamin E) as a standard substance. Different fruit juices were measured for their ORAC value.

View the application note here: ORAC Assay Performed on the POLARstar Omega and PHERAstar FS microplate reader

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