Thursday, January 12, 2012

Next-Generation Instrument Discovers Low Affinity Inhibitors of the Inositol Phosphate Pathway

BMG LABTECH's EJ Dell has just published an article in the current issue of IPI - International Pharmaceutical Industry which compares BMG LABTECH's PHERAstar FS HTS microplate reader to a high-performance charged coupled device- (CCD-) based camera imaging instrument for both speed and assay quality.

Summary
The second messenger inositol 1,4,5- triphosphate (IP3) was screened in an antagonist campaign for low affinity compounds using two different HTS microplate readers with different detection technologies. Utilising the IP-One HTRF® assay, a next-generation photomultiplier- (PMT-) based instrument was compared to a high-performance charged coupled device- (CCD-) based camera imaging instrument. In both speed and assay quality, the PMT reader clearly outperformed the CCD imaging reader. Using this lower response second messenger assay shows how the CCD imaging reader does not reveal lower affinity compound ‘hits’, thus making the PMT-based HTS reader the right choice for this and other screening paradigms.

To read the entire article, click here: http://www.bmglabtech.com/db_assets/applications/downloads/others/next-generation-instrument.pdf

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