Tuesday, December 27, 2011

Did you know that the rare earth metals used in time-resolved fluorescence assays (usually europium or terbium) do not fluorescence by themselves?

These lanthanides need to be “caged” in a macromolecule that helps the element to absorb and emit energy in the form of fluorescence. For discovering this long-lived fluorescence property of the Eu3+ cryptate, Professor Jean Marie Lehn was awarded the Nobel prize in Chemistry in 1987. The practical use of this research has been TR-FRET assays such HTRF®.

For more information on the chemistry used in HTRF assays, please visit Cisbio’s website here: http://www.htrf.com/technology/htrftheory/htrf_chemistry/ 

BMG LABTECH has a wide range of microplate readers that can be used to read such time-resolved fluorescence assays. In fact, the Gold-Standard microplate reader for HTRF® is the PHERAstar FS. For more information on the PHERAstar FS, watch our video at: http://www.youtube.com/watch?v=91usBQHPLyA


HTRF is a registered trademark of Cisbio Bioassays

No comments:

Post a Comment

Please let us know what you think...