Time Resolved-Fluorescence Resonance Energy Transfer (TR-FRET) is a preferred fluorescent assay format in drug discovery laboratories. TR-FRET assays are less susceptible to compound interference than other assay formats and may be applied to multiple target classes. To support this technology focus, Life Technologies has developed LanthaScreen® biochemical and cellular assays to measure kinase activity, compound binding, and post-translation modification events, such as phosphorylation and acetylation of substrates, in cell signaling. LanthaScreen® assays utilize terbium and europium chelates as FRET donors. Terbium chelate is used as a donor for fluorescein and GFP and europium chelate is a donor for Alexa Fluor® 647.
For more information on the LanthaScreen® Assay visit: http://www.bmglabtech.com/technology/lanthascreen.cfm
LanthaScreen is a registered trademark of Invitrogen Corp.